Induction of apoptosis in p16INK4A mutant cell lines by adenovirus-mediated overexpression of p16INK4A protein
نویسندگان
چکیده
منابع مشابه
Induction of apoptosis in human tumor cell lines by platelets
Introduction: It has been reported that platelets can eradicate tumor cells in vitro, although the mechanism of this effect has not been determined. The effect of platelets on the induction of apoptosis in tumor cells is largely unknown. Materials and methods: To investigate this effect, two human hematologic cell lines, K562 and Daudi, were independently faced with unstimulated and thromb...
متن کاملEffect of 5-aza-2′-deoxycytidine on p16INK4a, p14ARF, p15INK4b Genes Expression, Cell Viability, and Apoptosis in PLC/PRF5 and MIA Paca-2 Cell Lines
Background: Mammalian cell division is regulated by a complex includes cyclin-dependent kinases (Cdks) and cyclins, Cdk/cyclin complex. The activity of the complex is regulated by Cdk inhibitors (CKIs) compressing CDK4 (INK4) and CDK-interacting protein/kinase inhibitory protein (CIP/KIP) family. Hypermethylation of CKIs has been reported in various cancers. DNA methyltransferase inhibitors (DN...
متن کاملP16INK4a expression adenovirus vector to suppress pancreas cancer cell proliferation.
The prognoses of pancreatic cancer patients have been miserable even after radical surgery, and adjuvant therapy is necessary to improve the surgical results. p16(INK4a) (p16) is tight-binding and inhibitory protein for cyclin-dependent kinase 4 to induce G1 arrest of the cell cycle. p16 gene deletion is frequently identified in human pancreas cancer. The impaired gene function of p16 might be ...
متن کاملinduction of apoptosis in human tumor cell lines by platelets
introduction: it has been reported that platelets can eradicate tumor cells in vitro, although the mechanism of this effect has not been determined. the effect of platelets on the induction of apoptosis in tumor cells is largely unknown. materials and methods: to investigate this effect, two human hematologic cell lines, k562 and daudi, were independently faced with unstimulated and thrombin-ac...
متن کاملp16INK4A adenovirus-mediated gene therapy for human head and neck squamous cell cancer.
Inactivation of the tumor suppressor gene p16INK4A is the most common genetic alteration in human head and neck squamous cell cancer (HNSCC), making it an ideal target for gene replacement. We constructed a replication-defective, recombinant adenovirus capable of directing a high level of p16INK4A protein expression (Ad5-p16) to investigate its benefit in treating HNSCC. Initial in vitro experi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cell Death & Differentiation
سال: 2000
ISSN: 1350-9047,1476-5403
DOI: 10.1038/sj.cdd.4400703